THPDP054
MAX IV Laboratory, Lund University, Lund, Sweden
* Ursby T. et al. "BioMAX - the first macromolecular crystallography beamline at MAX IV Laboratory." Journal of Synchrotron Radiation 27, 1415 - 1729, (2020).
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THPDP054 |
Fast, Fully Automated Continuous Energy Scan at the Biomax Beamline at Max IV Laboratory | |
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BioMAX is an X-ray macromolecular crystallography (MX) beamline* at MAX IV Laboratory that delivers an X-ray beam with a photon flux of up to 1e13 ph/s. The photon energy at the beamline can be easily adjusted between 6 keV and 24 keV. At MX beamlines Single- and Multi-wavelength Anomalous Dispersion (SAD and MAD) methods are used for experimental phasing to reconstruct the macromolecular structures. To be able to benefit from these techniques, it is imperative for an MX beamline to have a fast and automated energy scan routine. This contribution reports on the newly implemented continuous energy scan procedure at BioMAX. The scan routine performs a synchronous motion of the undulator and monochromator motors to continuously scan the energy while measuring the fluorescence from the sample as the energy changes. The data acquisition during the scan is triggered by the actual energy value which is monitored throughout the scan at 1 MHz rate. The energy scan routine is fully automated and minimizes the radiation damage on the sample during the measurements. The scan itself is as short as one second making the overall procedure a factor of five faster than a conventional step scan.
* Ursby T. et al. "BioMAX - the first macromolecular crystallography beamline at MAX IV Laboratory." Journal of Synchrotron Radiation 27, 1415 - 1729, (2020). |
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Poster THPDP054 [4.700 MB] | |
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